LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Acquisition Tab
Systems
90 M60-1-0025
e
02/2010
5.2.12.5
Correction
The
Refractive Index Correction
considers the
different refractive indices between the immersion
medium of the objective (n') and the embedding
medium of the specimen (n), which can be set
between 0.5 and 3.
'
n
n
Ratio
=
Auto Z Brightness Correction
(Fig. 81) allows setting of different
values for the scan parameters
Detector Gain
,
Ampl. Offset
,
Ampl. Gain
and
Laser Attenuation
of up to 10 freely selectable slices
within a Z-Stack. During the scan procedure, the values of these parameters are automatically linearly
interpolated between the neighboring positions within the Z-Stack. This function can be activated and
deactivated with the check box
Use Correction
.
Add
takes the above mentioned parameters and
correlates them with the current Focus Position which is then added as a
Position
in the list (for example
the first slice of a stack). Adding a new position with a defined set of channel parameters cannot be done
while scanning. The positions don't need to be listed in a specific order, the program will sort the values
automatically in the background.
Move to
moves the actual focus to the highlighted
Position
in the list
without scanning. Starting a
Continuous
scan then allows setting or correcting the parameters and
storing them again hitting
Add
. Highlighted positions can be removed (
Remove
) or the whole list can be
removed (
Remove all
). The interpolation between the
Positions
in the list can be extrapolated (check
the box next to
Extrapolate
) to the actual first and last slice of a Z-Stack if those are not part of the
range of the
Positions
in the list.
When
Enable Test
is active, scanning at single positions of the stack or while focusing through the stack
is done using the settings of the
Position
list or the interpolated values calculated for the respective
focus position. This allows controlling the settings before starting the actual acquisition of the stack.
Fig. 81
Auto Z Brightness Correction
