LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Acquisition Tab
Systems
56 M60-1-0025
e
02/2010
Diode lasers
The LSM 710 and LSM 780 systems can be
equipped with Diode 440 and / or Diode 405 lasers
that can be used in continuous wavelength (cw)
mode or in pulsed (ps) mode (Fig. 44).
Selection of the mode is available in the Mode
pull down menu in the
Laser properties
menu
(Fig. 45).
You can select between
Continuous Wave
or
Pulsed - 20 MHz,
Pulsed - 50 MHz
and
Pulsed -
80 MHz
mode.
The diode lasers are directly modulated. They can
be further attenuated by ticking the
ND04
check
box, which will place a neutral density filter in front
of the lasers
Please note that pulsed modes were specifically
designed for Fluorescence Lifetime Microscopy
(FLIM) and should be preferentially used for this
application. Since laser powers in the pulsed mode
are substantially less than in the cw mode, the
pulsed mode is not suitable for bleaching
experiments. Also signal-to-noise ratios in intensity
imaging are less in the pulsed mode compared to
cw operation, hence the cw mode should be
chosen for high quality imaging.
Fig. 44
Laser menu with Diode 405-30 activated
Fig. 45
Mode pull down menu
