02/2010
21
Scanning a Z stack
•
Select
Z-Stack
in the main
tools area.
•
Open the
Z Stack
tool in the
Left Tool Area
.
•
Select
Mode First/Last
on the top of the
Z-Stack
tool.
•
Click on the
button in the
Action
Button
area.
A continuous XY-scan of the set focus position will
be performed.
•
Use the focus drive of the microscope to focus
on the upper position of the specimen area
where the Z Stack is to start.
•
Click on the
Set First
button to set the upper
position of the Z Stack.
•
Then focus on the lower specimen area where the recording of the Z Stack is to end.
•
Click on the
Set Last
button to set this lower position.
•
Click on the
button to set number of slices to match the optimal Z-interval
for the given stack size, objective lens, and the pinhole diameter.
•
Click on the
Start Experiment
button to start the recording of the Z-Stack.
When a multi-dimensional acquisition tool is not selected, the respective tool and its set parameters
are not included in the multidimensional image acquisition. If no multidimensional tool is activated,
the
Start Experiment
button is grayed out and only single images can
be scanned.
Fig. 26
Z Stack tool
