MULTIPHOTON LASER SCANNING MICROSCOPY …
LSM 710 and LSM 780
Carl Zeiss
Non Descanned Detection (NDD)
Systems
10 M60-1-0025
e
02/2010
Fig. 9
Potential filter equipment for a three channel NDD set up on Axio Examiner starting with an
NDD module type A or B
Adding NDD channel 3, 4 or 5 to an existing set up does require rearranging and swapping of individual
filters within and between single P&C cubes for the systems with Axio Examiner or Axio Imager.
For example, to add a fourth channel to the cascade shown in Fig. 10 with a filter combination to
additionally detect an emission signal in the far red range the filters have to be rearrange the following
way:
Fig. 10
Emission filters that need to be interchanged when upgrading a three channel to a four
channel system using the standard filter set.
The emission filters in the filter cube for the green channel in front of channel three is interchanged with
the emission filter of the filter cube for the orange signal which sits in front of channel one.
The cube with the mirror (now detecting the orange signal) is then placed in the new additional
NDD module.