LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Processing Tab
Systems
140 M60-1-0025
e
02/2010
5.3.14.4
Shading Correction
Shading Correction is a tool to manipulate images
which are unevenly illuminated over the field of
view. This works with single images, time series,
Z-Stacks or tiled images. Click on Shading
correction to open the interface for using that
function.
Select
the image (or image series, tiled image or
Z-Stack) for which the shading correction should
be applied by clicking
Select
next to
Input image
.
When clicking
Apply
the system calculates a
reference image from the input image(s) which is
used to correct the input image(s). The corrected
image is displayed in the center area screen.
In addition the calculated
reference
image
can be
stored and used for further shading corrections of
other images. Therefore the check box in the lower
part of the tool box muss be checked. Specify the
folder where the image should be stored in the
text box next to
Path
.
Browse
opens the windows
folders from which you can select. A suggestion
for the name of the image is made based on the
original name of the image. Change this if needed.
This reference image can then be loaded and
selected as reference image for the shading
correction of subsequent images, which are then
processed much quicker.
The reference image must be taken from an image
that was acquired with identical settings for
objective, zoom, image format and orientation.
Fig. 152
Shading Correction interface
