CONFOCOR
3
Carl Zeiss
Options
ConfoCor 3
132 M60-1-0025
e
02/2010
Hysteresis correction of Scanning mirrors
In general, there is no need to compensate
between defined positions and the actual
measurement positions using the scanning mirrors.
However, the precise arrest position of the mirrors
depends on the used scan zoom and speed and
small offsets can occur and offsets may exist due
to scanner hysteresis (Fig.
145). To adjust for
differences, the compensation sliders of the
bidirectional Scan can be adjusted to decrease the
offset (Fig. 146).
To compensate for Scan Zoom and Scan Speed go
to the
Acquisition
Mode
too of the LSM
Online
Acquisition
tool group. Use a sample with high
contrast, like "Convolaria", and focus. Activate
Bidirectional Scan
(double arrow) under
Direction
(Fig. 147). During scanning press the
Auto
button. The system will adjust automatically
the
Scan Corr X
and
Y
values. Alternatively you
can do the compensation manually.
Fig. 145
Scanner positioning before
hysteresis correction
Fig. 146
Scanner positioning after hysteresis
correction
Fig. 147
Pixel Depth, Scan Direction & Scan
Average window