CONFOCOR
3
ConfoCor 3
Models
Carl Zeiss
02/2010 M60-1-0025
e
69
D.3 Flow
Flow signifies active transport either via cytoplasmic movement or directed transport.
If flow occurs in the absence of translational diffusion, the term is defined as follows:
2
)
(
⎟⎟
⎠
⎞
⎜⎜
⎝
⎛
−
=
f
e
G
f
τ
τ
τ
(8m)
In the presence of translational diffusion, the term alters to:
)
(
1
)
(
2
)
(
d
f
e
G
f
τ
τ
τ
τ
τ
+
−
=
(8n)
with
τ
f
representing the average residence time for flow and
τ
d
the diffusion correlation time.
Note, in the ConfoCor software, the correct term is automatically loaded in dependence on the absence
or presence of a translational term.
With the knowledge of the lateral radius
ω
r
given as a fixed value, the software allows to fit directly to
the velocity v instead of the average residence time. The following relation exists:
f
r
v
τ
ω
=
(8o)
.
