LSM 710 and LSM 780
TOOLS, ADDITIONAL SOFTWARE
Systems
3D for LSM
Carl Zeiss
02/2010 M60-1-0025
e
47
Method
The
Input
sequence defines the data to be reconstructed. If it is a multichannel sequence one or all
channels can be selected for the reconstruction.
Output
sets the name of the result image (sequence). If the sequence exists it is overwritten. Pressing the
button
New
will generate a new name (number). The size of the sequential images in
Output
is
determined by the size of the sequential images in
Input
.
Number of Views
determines the number of reconstructions which should be computed. The radio
buttons
Start
and
End
define which angle settings are currently shown. A definition for the angle
End
is
only necessary if
Number of Views
is higher than 1. If this is true the result sequence will get views from
the
Start
to the
End
angle definition. The other reconstructions are determined through the linearly
interpolated intermediate angles.
The direction of view is determined from the angles as follows:
The angle
Angle Z
determines the rotation of the direction of view on the Z-axis. The angle
Angle Y
determines the rotation of the direction of view on the Y-axis that has been rotated by the angle
Angle Z
. The angle
Angle X
determines the rotation of the direction of view on an X-axis that is rotated
by
Angle Z
and
Angle Y
.
Channel
defines if the following parameters are valid for
All
or just for one. Defining the opacity for the
channels independently is useful when the brightness and contrast of the channels differ too much.
Threshold
defines the range with no opacity. It is completely transparent. The range starts at grey
value 0.
The length of slope is defined by
Ramp
. The maximum opacity value is set with the parameter
Max.
Opacity
. This range ends at the maximum grey value. The
Opacity Table
shows the grey value
histogram of Input with the opacity definition as a red line.
When
Auto Update
is selected, the reconstruction is updated automatically whenever a parameter is
modified (except
Input, Output
, or
Number of Views
).
Show Cube
defines whether a wire frame cube
is shown in the
Display window
or not.
Application
1.
This method can be applied, if the structures in the Input sequence are unsharp so that objects are
poorly defined by their grey value.
2.
In this case, the Opacity Table is defined as a ramp. Low grey values have weight 0 to suppress the
background voxels. The opacity rises with increasing grey values, depending on the parameter Ramp.
The value of Max. Opacity defines the weight of the high grey values. High grey values above a
threshold have weight 255 to show the "object" voxels unsuppressed. Of course a smooth step can
be used.
3.
The result is a display with inside structures shining through. A 3D impression can be obtained by
rendering with several view directions.
4.
In contrast to this, a voxel renderer like the gradient renderer would display only the surface of objects
that are defined by grey value-thresholds. This surface would appear shaded as if illuminated by a
light.
5.
The method can also be applied to visualize pronounced structures within other enclosing structures, if
the structures have different grey value ranges.
6.
In this case, the Opacity Table is defined as a step. Low grey values (background) have weight 0. High
grey values (inside structures) have maximum weight.
