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CMI | SOP003_rev1.0 

 

CMI STANDARD OPERATING PROCEDURE

 

 

 

 

Confocal Raman Microscope 

Physiology Ground floor research lab 

 

 

 

CMI document ID: SOP003

 

 

 

 

 

 

CONTACT INFORMATION: 

 

Peter Owens: 091 494036 (office) 

[email protected]

 

  Jennifer Connolly: [email protected]  

   

 
 
 
 

REQUIREMENTS FOR EQUIPMENT USAGE:

 

 

1. CMI user 

2. Completion and signing of Microscope Safety Checklist 

3. Certification by Peter Owens or Jennifer Connolly 

 
 

REVISION LOG

 

 

 

 

Revision

 

 

Date

 

 

Author

 

 

Changes

 

 

1.0

 

 

12/5/14

 

 

P. Owens

 

 

Initial draft

 

 
 

1.

 

Purpose 

 
1.1.

 

This document specifies the work instructions for the CMI Witec Confocal Raman microscope located in 

the ground floor Physiology lab, Quadrangle building.  If you see an area where more clarification is 

needed, if additional information is needed, or if you have suggestions on how to make this guide more 

useful in the lab, please contact the CMI. 

 

1.2.

 

Note that this document is not a detailed instrument manual and does not intend to be one. For 

detailed questions or if anything unusual happens with the system, please refer to the manuals 

present in the lab, or ask CMI personnel for help. 

 
 

 

Summary of Contents for SOP003

Page 1: ...hor Changes 1 0 12 5 14 P Owens Initial draft 1 Purpose 1 1 This document specifies the work instructions for the CMI Witec Confocal Raman microscope located in the ground floor Physiology lab Quadran...

Page 2: ...ation in a Laser safety course is strongly recommended for operating this system 3 2 Witec Alpha 500 manual hardcopy in the lab soft copy on the instrument PC and training notes 3 3 The CMI access pol...

Page 3: ...quire a Raman spectrum of most substances in seconds via Charge Coupled Device detectors CCDs that have a wide dynamic range for users to select the appropriate exposure time for their sample Dependin...

Page 4: ...with the system folders located on the shelf above the equipment 7 Start Up Procedure 7 1 Book equipment on CMI web booking system 7 2 Turn on lasers or individual laser if only one is to be used For...

Page 5: ...o be warmed up for at least 30 mins before acquisition while warming up alignment of the microscope can be done 7 7 Click on the Witec Project Control to start the raman software interface 633nm laser...

Page 6: ...tness to 100 in the software Illumination Make sure to see some intensity changes in the video image check it by opening and closing the aperture stop A at the microscope Typically keep this just slig...

Page 7: ...t directed to eyepiece camera Eyepiece camera Aperture stop Field stop Up down toggle between z control xy stage control X Y Stage controls Z Stage controls Z stage moves away from objective Z stage m...

Page 8: ...tching between wavelengths but can be possible as a quick check just to swap in the fibre cables Note Take extreme care when handling the fibre cables as they can be damaged very easily Ensure that th...

Page 9: ...or cells vibrational bands of C H stretching around 700 and 1000 wavenumbers can be used for mapping 9 4 After selecting the large area scan images with better contrast between cells and nanoparticles...

Page 10: ...should be 60 o C 11 Shut Down Procedure 11 1 You can save the experiment parameters for your own scanning recipe by clicking Save Project in File 11 2 Close the laser shutter switch off the laser powe...

Page 11: ...mple on the microscope Demonstrate finding the sample optimizing acquisition and taking spectra Demonstrate changing the fibre cable during the measurement session Demonstrate finishing and shutting d...

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