CMI | SOP003_rev1.0
6
8
Calibration
8.1
Use the silicon wafer for calibration (accepted Raman peak at 521 wavenumbers)
8.2
Choose objective to use and switch to brightfield mode (with edge filter and dichroic mirror off);
beam splitter should be in Bright Field (BF) mode (Pos 1)
8.3
Set the detector knob, at the side of binocular, to the eyepiece mode (and not the camera mode)
8.4
Set the brightness to 100% in the software (‘Illumination’). Make sure to see some intensity
changes in the video image; check it by opening and closing the aperture stop (A) at the
microscope. Typically keep this just slightly open, unless for dim samples/dic imaging.
Laser coupler
Slide rightwards(follow
arrow direction)
Options are block:
Open:
Either open , block for
633nm and 785 nm
can be chosen. All
other positions have
no filter , used for
brightfield.
Filter turret
Position 1: Brightfield
imaging
Position 2: DIC for
water dipping lens
Position 3: DIC for all
other lenses
Position 4: Raman
imaging, spectra
