Approving a Calibration
Introduction
System Setup
IMMAGE 800 Operations Manual A11403
Page 5-102
September 2016
Approving a Calibration
Introduction
After the calibration run, the instrument response (IR) for each calibrator replicate is
generated. The IR versus calibrator set point concentration, as well as curve-fit, is
displayed as a plot using various curve-fit models. The calibration model can be
selected for the UDR. The model and associated calibration parameters are saved for
the reagent lot numbers defined for this UDR, or until another calibration is requested.
The four models available are First Order Polynomial, Second Order Polynomial,
Third Order Polynomial, and Four Parameter Logistic.
Curve-Fit Model Descriptions
The following table describes the four curve-fit models. Refer to Figure 5.47 to Figure
5.53.
Table 5.14
Curve-fit Models
Model
Minimum number
of calibrator levels
to plot graph
Formula
Parameter
Displayed
First Order
Polynomial
4
A
B
Second Order
Polynomial
5
A
B
C
Third Order
Polynomial
6
A
B
C
D
Four Parameter
Logistic
6
A
B
C
D
A011363L.EPS
y = A + Bx
A011364L.EPS
y = A + Bx + Cx
2
A011365L.EPS
y = A + Bx + Cx
2
+ Dx
3
A011366L.EPS
y =
+
D
A - D
1 +
C
x
B
⎧
⎩
⎫
⎭
