LSM 880
Center Screen Area / Image Containers - Display and Image Analysis
ZEISS
10/2014 V_01
000000-2071-464
503
6.18.1
Tools in the FRET View Options
Control Block for Acceptor
Photobleaching
FRET tab (Fig. 695):
As
Method
for FRET Analysis,
Acceptor
Photobleach
is automatically selected in the drop
down list.
Within this tab the overlay regions present in the
image (defined as bleach, background or control
region of the bleach experiment) can be activated
(The drawing tools correspond to the drawing
tools described in section
). Additional
regions can be defined for analysis as well.
The checkboxes
Numbers
and
Measure
refer to the overlay regions and annotate the number of the
region and its area to the overlay in the image.
Export
allows the resulting FRET image to be saved as a separate image file.
The regions defined can be set as
Object
(Region of which FRET should be calculated) or
Background
(from which the threshold for the image analysis is deduced from and automatically used for analysis and
displayed in the
Threshold tab
).
Regions and the assigned status as Object or Background can be selected or deselected for the individual
analysis using the corresponding check box under
Enabled
.
To start analyzing the
Analyze
button must be clicked once. If all further changes shall be effective
immediately the
On the fly
checkbox can be checked.
Parameter tab (Fig. 696):
Various parameters of the image series can be set
in this tab.
The image of the Donor and the Acceptor are
assigned to the appropriate channel using the
drop down menu next to
Donor ch.:
and
Acceptor ch.:
The frame of the time series after the bleaching
event (
Bleach after frame
) is set using the slider
next to it or by editing the field next to the slider. If
only one bleach event was performed, this defines
the frame before and after the bleaching
respectively for analysis.
In case of a bleach experiment with repeated bleaching time points the checkbox
Bleach Repeat
must
be checked. This results in activation of the
Donor post-bleach Frame
slider and field. Here the frame
can be chosen to provide the data of the donor intensity after bleaching (D post) for analysis.
Fig. 695
FRET View Options Control Block,
FRET tab
Fig. 696
FRET View Options Control Block,
Parameter tab
Summary of Contents for LSM 880
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