Zeiss LSM 880, Operating Manual

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CHAPTER 5 - MULTIPHOTON LASER SCANNING …
ZEISS NLO (Non Linear Optics) Laser for LSM LSM 880
2 000000-2071-464 10/2014 V_01
1 NLO (Non Linear Optics) Laser for LSM
The LSM scanning module is operated with a Titanium-Sapphire-Laser and optionally in combination with
an optical parametric oscillator (OPO) to perform Multiphoton Microscopy. Depending on the type of
laser the following operation procedures apply:
•
The operation of software controlled lasers (Chameleon laser from Coherent or Mai Tai laser from
Spectra Physics) is integrated into the LSM software.
−
Both lasers should be kept in Standby mode for daily or weekly operation. The cooling of the laser
must not be shut down during stand by. Only in case of more than two weeks without operation
the laser should be shut down completely. This has to be performed according to the operation
manual for the laser provided by the manufacturer. The shut down and the start procedure can
take up to one hour.
−
Specific for the operation of Chameleon lasers (Coherent; for further details please refer to the
operators manual of the laser):
The key on the front panel of the laser power supply is used for switching between Standby mode
and Operation mode. The Chameleon laser has to be in Operation mode and
On (see sections 1.1
and 1.2) to be able to pump the OPO.
The laser has to be in Operation mode in order to control it with the LSM software.
−
Specific for the operation of Mai Tai lasers (Spectra Physics; for further details please refer to the
operators manual of the laser):
The laser is put to
Operation
mode or Stand by mode using the LSM software. Therefore no
further operation of the laser via the laser power supply and electronics unit is needed. The key on
the front panel of the laser power supply can also be used to put the system from Standby to ON. It
is also an interlock key. When the key is removed the system cannot be operated. The key needs to
be in ON position to be able to control the laser with the software. The power switch on the laser
electronics has to be kept
On
for leaving the laser in Standby.