ZEISS
Left Tool Area and Hardware Control Tools
LSM 880
142
000000-2071-464
10/2014 V_01
5.2.15.1
Performing a Z-Stack Using
First/Last Mode
Press the
First/Last mode
button to open the
First/Last definition
panel.
Press the
Continuous
action button and focus
onto the specimen.
While scanning change the focus using the
Acquisition Parameter -
Focus
tool, the
Z-Stack
Navigation
(Fig. 193) or use the focus drive of the
microscope.
When you reach the position within the specimen,
where you would like to start the stack, hit
Set
First
and this position (in µm referring to the 0
position of the Z drive) will be taken as the first
position in the stack.
Move the focus into the other direction until the
end position within the stack is reached, then hit
Set
Last
. These focus positions are then indicated
next to
Set First
and
Set Last
buttons. The
Range
display box shows the total height of the stack in
micrometers.
The
Slices
input box displays the number of slices of the stack. This number can be edited by typing or
using the arrow keys. Changing the value for
Slices
automatically adjusts the
Interval
in a way that the
total
Range
for the stack is maintained.
The
Interval
input box displays the z-distance (interval) between slices in
µ
m
. This number can be edited
by typing or using the arrow keys. Changing the value for the
Interval
automatically adjusts the
Number
of Slices
while keeping the total
Range
for the stack within the possibilities of fitting the calculated
number of slices into the Range. The total Range may therefore vary to higher and lower numbers
around the
initial value of the Range
.
Pressing the
Optimal
button (only available in
SIM
and
LSM
imaging modes, greyed out in
WF
and
Laser WF imaging
modes) sets
Interval
to match the Nyquist criteria (2 fold over sampling) and
accordingly adjusts the
Slices
to keep the
Range
within the limits defined by
Set First
and
Set Last
. The
optimal interval is displayed in the button in
µ
m
.
Within the
Keep
display field (only available in
Show all
mode) you have the option to switch between
Keep
Interval
and
Keep Slice
. If
Keep Interval
is checked, the number of slices is adjusted to a new
Range
defined pressing
Set First
or
Set Last
. Likewise, when
Keep Slice
is checked, the value for the
Interval
between the slices is adjusted to a new Range defined by pressing
Set First
or
Set Last
.
Fig. 193
Z-Stack Navigation appearance for
the LSM image mode
Summary of Contents for LSM 880
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