Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Center Screen Area / Image Containers - Display and Image Analysis ZEISS
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Available tools in the FRAP View:
Channels
Select single channels or all channels for analysis in the general Dimensions view
options control box.
Kinetic Model / Fit
formula
Pull down menu to select the mathematical model (mono or double exponential
model) for fitting the data.
Analyis Areas /
Regions of Interest
(ROIs)
The
FRAP
View Options control block includes drawing tools that work
identically to the graphics view options control block. ROIs from both control
blocks can be combined.
Background
Region
Check box in the list of ROIs: Mark the region of interest which represents the
mean background intensity to be used for data correction.
Reference Region Check box in the list of ROIs: Choose and mark the ROI which represents the
fluorescence intensity of a reference cell that has not been bleached. The mean
intensity within that region is used to correct the data at each time point for any
bleaching artifact that occurred during the imaging process.
Combine Regions
Group 1, 2, 3
Check boxes in the list of ROIs which allow to choose more than one ROI for
analysis and to group them according to the experimental set up.