Zeiss LSM 880, Operating Manual

Page: 224 / 690

CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
218 000000-2071-464 10/2014 V_01
You can choose between symmetric rotation
(1 exponential term) and asymmetric rotation
(double exponential term) by selecting / deselecting
the
Asymmetric check box (Fig. 285).
If the
Asymmetric
box is checked, you can define
relative amplitudes and rotational frequencies
(note, these parameters are no fit values and have
to be defined by the user).
Relative amplitudes are defined by selecting the
excitation polarization (
linear polarized
or
unpolarized ) and the emission detection
( parallel , perpendicular or all ), by pressing the
corresponding tabs, which will result in the
corresponding values displayed in the
c1
and
c2
display boxes. Alternatively, if others is activated,
you can type in user defined values.
Rotational frequencies can be selected by pressing
the
Default tab to load the default values. The
corresponding values will be shown in the
r1
and
r2 display boxes. By pressing the other tab, user
defined values can be entered.
The description box gives information on the
rotational diffusion terms.
Fig. 285 Rotation Settings panel,
Asymmetric rotation checked