Zeiss LSM 880, Operating Manual

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CHAPTER 4 - ANNEX
LSM 880 Courses on "How to Operate the System … ZEISS
10/2014 V_01 000000-2071-464 5
2 Courses on "How to Operate the System in an Optimized Way"
ZEISS is offering training courses on how to operate the system in an optimized way.
−
Courses are held in our application center in Jena, Germany.
−
Courses are held in English or German language, respectively.
Check out:
www.zeiss.de/lsm
for the latest dates and ask your Zeiss representative for a quotation on courses.
3 Beam Path Configuration Guide for Fluorescence Microscopy
3.1 Optical Elements in the Configuration Control Window
All wavelength values are given in Nanometer [nm].
3.1.1 Main Dichroic Beam Splitter (MBS)
•
A
MBS XXX/YYY
deflects the indicated laser lines onto the specimen and allows the emitted
fluorescent light to pass through.
Example: MBS 458/514, MBS 488/543/633
•
A
MBS XXX+
is a special type of a main dichroic used for IR multiphoton excitation. The
MBS 690+
for example, deflects laser light with a wavelength longer as 690 nm onto the specimen and allows
fluorescent emission light in the visible range below 660 nm to pass through.
Example: MBS 690+
•
A MBS XXX/YYY+ is a combination of a MBS XXX and MBS YYY+ used for simultaneous IR
multiphoton and single photon excitation.
Example: MBS 405/710+.