Zeiss LSM 880, Operating Manual

Page: 168 / 690

CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
162 000000-2071-464 10/2014 V_01
5.2.20 Multidimensional Acquisition –
Positions
When the
Positions
tool tab is expanded the
Positions tool becomes available (Fig. 234).
This function comprises two main applications.
First, it is used to take images of a specimen at
freely defined positions (
Positions List mode).
Second, it is used to image specimens, typically
cells, which are grown in sample carriers like plates
with a set number of wells (
Sample Carrier
mode).
5.2.20.1 Position List
Press the
Position List
button to obtain the
Position List control panel (Fig. 235)
Clicking the
Add
button adds the current position
and its coordinates to the list of positions.
Displayed are the number of the positions, their
x
(
μ
m) , y (
μ
m) and z (
μ
m) coordinates and also
their
offset
values (µm). See the description of
Setting individual offset values for positions
in Position List mode
(below) and also the tool
description of Focus Devices and Strategy for
details on offset values (section
Acquisition
Parameter – Focus Devices and Strategy ).
Highlighted positions can be removed by pressing
the
Remove button. Alternatively the whole list
can be deleted by pressing the
Remove All
button.
It is also possible to move the stage to the
highlighted position by clicking the button
Move to
. The order of positions in the list can be modified by
pressing the buttons Up or Down .
You can save and load pre-saved positions by pressing the
Save…
and
Load…
buttons, respectively,
which will open Windows Explorer.
The list of positions is consistent with the positions defined in the
Stage tool.
When the list of positions is deleted in the
Position list
, it is also deleted in the
Stage
tool. This
is not the case the other way round.
It is recommended to save the position list as relative positions. The format "Positons list files
(relative)" should be selected in the Windows Explorer. This format allows to reuse the positions
for other experiments later on. Just make sure that the reference position for the list (for
example the position 0) matches with the actual zero position in your sample. Start with
positioning the sample at the desired zero position and set the stage to zero before loading the
position list.
Fig. 234 Positions tool
Fig. 235 Positions List control panel