Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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Ratio Imaging Configuration
In the Show all mode of the Imaging Setup tool
two additional ratio imaging channels
R1
and
R2
can be defined. These channels are displayed in
addition to the maximum of 8 active imaging
channels. They provide the possibility to display the
ratio between two of the active channels.
A click on the arrow next to
Ratio
in the lower
part of the
Imaging Setup
tool window (Fig. 114)
provides access to the ratio channel interface. A
maximum of two ratio channels can be activated.
These channels can display the ratio between two of the active imaging channels. The source channels
for calculating the ratio have to be selected from the drop down menu next to Source 1 and Source 2. As
an alternative to a second channel for the ratio imaging is also possible to choose the first images of a
time series of one channel for the ratio metric calculation (e.g. to calculate F/F
0
for single wavelength
dyes). The parameters for the calculation are set in the Channels tool (see section Acquisition
Parameter – Channels ).
A suitable color is assigned to each of the two Ratio Channels R1 and R2 , in the same way as for the
photomultiplier channels.
The ratio to be formed between the selected channels can be defined more precisely using one
of the four preset formulas in the
Channels tool window after a click on the relevant ratio
button (e.g.: R1) for online display of radiometric or single wavelength dyes. The Set by min/max
function (in
Channels
tool window) allows the definition of the display scaling according to the
expected minimal and maximal values.
Fig. 114 Ratio channels in the
Imaging Setup
tool