Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
72 000000-2071-464 10/2014 V_01
Smart Setup
displays its proposals side-by-side for
optimal comparison. The proposal selected for imaging is
highlighted. Key aspects of each proposal are displayed
as a bar chart representation.
Such bar charts contain information on the expected
emission signal (relative amount of detectable signal with
the proposed configuration), cross talk (relative
contribution of cross talk) and speed (acquisition speed
as percentage of acquisition speed in simultaneous multi-
channel recording).
The prediction of the emission signal considers both, the
efficiency of fluorochrome excitation and also the
detectable portion of the emission.
Smart Setup
displays a bar chart representation of
emission signal, cross talk and speed with each proposal.
The color-coding specified for the stains is used for
displaying the bar charts (Fig. 74).
Smart Setup displays the track configuration along with
normalized emission spectra of the chosen dyes (as
specified in the experiment configuration). Filled spaces
of the spectral curves illustrate the portion of the
emission detectable in a given proposal (Fig. 75). The
illustration also displays the order of track acquisitions
upon sequential multi-color recording. Track acquisitions
using long wavelength laser lines are performed first.
Pressing the button applies the selected
proposal as well as the motif. If a motif other than
Current
is selected, ZEN applies the proposal, the
scanning parameters of the motif and automatically
scans an image. Detector settings are automatically
adjusted by pressing the action button.
Fig. 74 Smart Setup bar charts
Fig. 75 Smart Setup tracks