Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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5.5.5.3 System Options – Hardware
Press the Hardware tab to open the Hardware
panel (Fig. 580).
Hardware lists parameters that, when their
respective check box is ticked, will set several
hardware defaults.
By activation of the
Lasers off on exit
check box
the lasers are automatically switched off when the
ZEN software is closed. The lasers are allowed to
cool for five minutes before they are switched off.
By ticking the
use argon laser line selection filter wheel (only applicable in LSM imaging mode) the
line selection filter in the LSM head is used for additional excitation laser line suppression.
If the
Keep raw data for photon count detectors
(only applicable for the
LSM imaging
mode) is
ticked, the actual photon counts will be assigned to each pixel and displayed in histograms. If no ticked
photon counts will be converted dependent on the color depth into grey values.
The Z-Stack
Waitstate function delays the image acquisition during time series or Z-Stacks in case
vibrations of the stage occur and are registered at the beginning of individual images. The default is off.
The delay is set to 350 ms. This can occur for specific image acquisition settings and is dependent on the
speed, the stage and the objective in use.
Online scanner calibration (OSC) (only applicable for LSM imaging mode) assures the calibration of
the scanners for uni- and bidirectional scanning is on as default setting. The default is on and it is
recommended to keep the calibration on at all times. If that function is not checked it might be required
to calibrate the scanners in x and y manually when bidirectional scanning is used for image acquisition.
Nonlinear bidirectional distortions especially at high acquisition speeds (10-15) can only be removed by
OSC.
The function will not be activated automatically upon starting the system if it has been
deselected before shutting down the system. When starting the software, a message will remind
you of activating the online scanner calibration.
Fig. 580 System Options – Hardware panel