Zeiss LSM 880, Operating Manual

Page: 353 / 690

CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
10/2014 V_01 000000-2071-464 347
5.4.17.2 Structured Illumination – Structured
Illumination
The
Structured Illumination
function allows automatic
or manual processing of a set of structured images
obtained by phase shift and rotation of the grid.
Select the
Structured Illumination
tab to select the
Structured Illumination Processing tool (
Fig.
479
). Activate the image you want to process in the
image container and press the
Select
button to load the
image. Then expand the Method Parameters tool
window to have access to setting parameters (Fig. 480).
You have to use the subdirectory Structured
Illumination
tab when selecting the image to
have aces to the Structured Illumination tools.
You will have no tools available is the directory
Structured Illumination tab is selected.
If a region of interest ( ROI ) is defined in the image, then
this ROI will be exclusively taken for image processing
and the rest of the image will not be processed.
You can instead of processing only one selected image
also load more images to be processed sequentially. To
this end press the
Run Batch button (Fig. 480). This will
open
Windows
Explorer from which you can select all
images you want to process. All selected files will be
displayed in the
Batch display window, which will
appear when the files are opened by Windows explorer.
The
Batch display window also shows the current state
of batch processing.
Fig. 479 Processing – Structured
illumination tool selected
Fig. 480 Structured Illumination –
Structured Illumination