Zeiss LSM 880, Operating Manual

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CHAPTER 2 - MACROS AND VISUAL BASIC
LSM 880 Visual Macro Editor ZEISS
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This block performs the Find function according to the main software.
This action block sets the Z-position according to the focusing procedure set
with the parameters available. The image generated displays the image or
image series aquired to set the focus position. The following properties are
available to define the auto focus function:
−
Mode/Precise: Uses fast XYZ-scan(s); the scan parameters can be
defined via Scan Parameters Action Block
−
Mode/Fast: Uses fast XZ-scan(s)
−
Method/Intensity: auto focus mode which searches for maximum
intensity.
−
Method/Contrast: auto focus mode which searches for maximum
contrast.
−
Range (µm): The system will perform the auto focus search within that
range.
−
Offset (µm): Defines the distance between the focus plane set by the
auto focus function (e.g. coverslip reflex) and the focal plane for
imaging.
−
Assign position to: Chose a variable to which the new position of the
focus plane set by the auto focus function (e.g. coverslip reflex) should
be assigned.
−
Assign offset to: Chose a variable to which the offset value should be
assigned.
Windows Action Blocks
This block has to be used if the actually scanned image(s) should be displayed.
For each Image Display block a new image window is opened. If not saved, the
image will be overwritten when the program flow is passing by again.
Load / Save / Action Blocks
The image(s) are stored to an image database. The database taken is either the
last one, that was open or the current open one. Clicking Read back updates
the database to be used for storing the image(s). In addition some other
parameters can be set according to the list of properties displayed.
Within the program flow already existing images can be loaded.
The database taken is either the last one, that was open or the current open
one. Clicking Read back updates the database to be used to load the image(s)
from. The images can be loaded by Index or by the name of the image.