Zeiss LSM 880, Operating Manual

Page: 214 / 690

CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
208 000000-2071-464 10/2014 V_01
a
r r
a
r
R
e c e c R
G
r r
−
⋅ + ⋅ ⋅
+ =
− −
1
) (
1 ) (
2 ,
2
1 ,
1
2 1
t
t
t
t
t
normalized
(8f)
with R
a
being the amplitude, c
1
and c
2
relative amplitudes, r
1
and r
2
frequency factors and
t
r,1
and
t
r,2
the
rotational diffusion times.
R
a
,
t
r,1
and
t
r,2
are fitted parameters; c
1
and c
2
as well as r
1
and r
2
are fixed values and must be user
defined.
Rotational frequencies take often the following values:
r
1
1
r
2
10/3
The relative amplitudes dependent on the polarization of the excitation light and the analyzer in the
emission beam path and are as follows:
Ex lin.
pol.
lin. pol. lin. pol. unpol. unpol. unpol.
Em parallel perpendicular all parallel perpendicular all
c
1
80 20/9 860/9 5/9 215/9 20
c
2
64/9 4 4 16/9 1 1