Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
96 000000-2071-464 10/2014 V_01
Imaging in Lambda Mode
(to be selected in the Imaging Setup tool).
The
Lambda Mode
is recording the overall emission
from the sample onto a wavelength-dispersive
element and is imaged on a 34-channel detector. All
34 photomultipliers of the detector cover a spectral
width of approximately 360 nm, a single PMT covers a
spectral range of 8.9 nm. In the
Lambda Mode
,
images, image stacks, or time series are recorded in a
wavelength selective way.
This new image format is called Lambda Stack. For
the acquisition of a Lambda Stack, the complete
fluorescence signal can be read out at once. The
settings of the beam path for the
Lambda Mode
scanning procedure with regard to the main dichroic
beam splitter and the QUASAR detector settings are
performed in the
Imaging Setup tool (Fig. 115).
The QUASAR detector is displayed for spectral
imaging if
Lambda Mode
has been chosen as
imaging configuration.
The
Imaging Setup
tool displays the laser lines, the
main (dichroic) beam splitter, and the spectral range
of the QUASAR detector.
You can change the settings using the following
function elements:
Activation / deactivation of the excitation wavelengths (check box) and setting of
excitation intensities (slider).
Selection of the main dichroic beam splitter (MBS) through selection from the
relevant list box.
Definition of number of PMTs out of the 34 PMT Detectors to fit the required
spectral range.
Access to the focus control (see section
Focus Control
)
Step width in spectral range (8.6 recommended).
Activation / deactivation of reflection.
Fig. 115 Imaging Setup tool window;
Lambda Mode activated