Zeiss LSM 880, Operating Manual, Page: 644 / 690

CHAPTER 5 - MULTIPHOTON LASER SCANNING …
ZEISS Non Descanned Detection (NDD) LSM 880
18 000000-2071-464 10/2014 V_01
For example, to add a fourth channel to the cascade shown in Fig. 13 with a filter combination to
additionally detect an emission signal in the far red range the filters have to be rearrange the following
way:
Fig. 13 Emission filters that need to be interchanged when upgrading a three channel to a four
channel system using the standard filter set.
The emission filters in the filter cube for the green channel in front of channel three is interchanged with
the emission filter of the filter cube for the orange signal which sits in front of channel one.
The cube with the mirror (now detecting the orange signal) is then placed in the new additional
NDD module.
The filter cube with the emission filter for the green signal is then placed in front of channel three. The
new additional filter cube for the red signal is placed into the two-channel NDD module in front of
channel one. The result is shown in the following figure:
Fig. 14 Potential filter equipment for a four channel NDD set up on Axio Examiner starting with an
NDD module type A or B