Zeiss LSM 880, Operating Manual, Page: 218 / 690

CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
212 000000-2071-464 10/2014 V_01
D.3 Flow
Flow signifies active transport either via cytoplasmic movement or directed transport.
If flow occurs in the absence of translational diffusion, the term is defined as follows:
2
) (






−
=
f
e G
f
t
t
t
(8p)
In the presence of translational diffusion, the term alters to:
) ( 1
) (
2
) (
d
f
e G
f
t
t
t
t
t
+
−
=
(8q)
with
t
f
representing the average residence time for flow and
t
d
the diffusion correlation time.
Note, in the FCS software, the correct term is automatically loaded in dependence on the absence or
presence of a translational term.
With the knowledge of the lateral radius
ω
r
given as a fixed value, the software allows to fit directly to
the velocity v instead of the average residence time. The following relation exists:
f
r
v
t
ω
=
(8r)
.