Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
178 000000-2071-464 10/2014 V_01
(1) Saving and deleting beam paths
You can save a beam path by typing in a memorable name intothe drop down menu selection box and
pressing the
Save button (floppy disc icon). If you save a beam path under the existing name you will be
prompted to confirm overwriting the old configuration. Press
Yes
to overwrite,
No
to keep the old
configuration.
You can delete a beam path by selecting it from the drop down menu and pressing the
Delete
(dustbin
icon) button.
Before saving an altered beam path a pinhole alignment should be performed. Pinhole positions
will be linked to the selected beam path and called up whenever that beam path is selected later.
(2) Loading a predefined beam path
You can select a saved beam path configuration from the drop down menu. The selected beam path will
than be loaded and shown with its settings in the displayed diagram. You can alter the beam path if
required.
Procedure to load and reconfigure an existing beam path:
•
Select a corresponding beam path from the Light Path drop down menu list box.
•
Change settings as required.
•
Save the configuration under the old name to overwrite the existing configuration or under a new
name to keep the old configuration.
(3) Define a new beam path
A new light path is defined by choosing the appropriate filters, dichroics and laser lines in the Light Path
diagram. Note that for BiG.2, filters are exchanged manually by exchanging the filter cube (consisting of
SBS, EF1 and EF2).