Zeiss LSM 880, Operating Manual

Page: 405 / 690

CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
10/2014 V_01 000000-2071-464 399
5.5.1.3 How to Adjust the Pinhole and
Collimator
Adjustment of pinholes can be performed
manually or automatically.
Temporary optimization of the adjustment:
The position of the pinhole relative to the detector
in terms of X-Y-Z coordinates contributes
substantially to image optimization.
Requirements to make the influence of pinhole
position changes on image brightness immediately
visible:
−
The image must be scanned by the
continuous scan method.
−
Select a fast scanning speed.
−
Measurement with Average Number 1 only
(no averaging of several measurements).
−
On the Channels tool in the LSM
Acquisition Parameter tool group select
the pinhole diameter so as to have the best
possible image contrast (see Fig. 556).
•
Open the Adjust Pinhole and Collimator tool
in the
Maintain
tool group in the
Maintain
panel.
•
Select the pinhole to be adjusted from the Name list box.
•
Use the Diameter slider to set the smallest possible size which produces a good, high-contrast image.
This setting changes the pinhole diameter.
Image optimization can be effected with the
Range Indicator
or in the
Line-Scan
mode.
Fig. 556 Channels tool