Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
110 000000-2071-464 10/2014 V_01
Frame Mode for Camera
When images are acquired with the camera only
Frame mode is available.
The following additional parameters and be set:
Objective, Frame Size, Format, Bit Depth,
Averaging and Offset
The exposure time is set in the Channels tool.
A list of available objectives is displayed in the
scroll down menu next to
Objective
. Choose one
from the list which is appropriate for imaging a
specific specimen. Pay attention to match the
refractive index of the objective with the refractive
index of the embedding medium of the specimen
if possible.
•
Select the
Frame Size
from the default sizes via
the drop down menu clicking the button
or type in the required values next to
X
and
Y
all the. Recommended setting to start with:
1300 x 1300. You can select between square
formats or free defined frame sizes.
Format
selects between a range of default camera
resolutions. The 5x5 binning mode can be used for
focusing without delay of the image display.
•
Select 8 Bit or 12 Bit Data Depth, i.e. 256 or
4096 gray values.
•
In the scan area it is possible to shift a sub region in the frame using the offset sliders if the Frame
Size
is set to a smaller value than the chosen
Format
sets it automatically.
•
The horizontal and vertical Offset can be set to zero individually clicking C .
•
Reset All Resets the Offset and the frame/sub region to the default value selected in Format .
•
Frame
mode is available for averaging.
•
Select the desired scan average method
Mean
or
Sum
.
•
Select the desired scan average from the available values 2 , 4 , 8 and 16 in the Number selection drop
down menu or
Continues
.
The greater the number of averages selected for Mean average Method , the better the image
quality will be; the scanning time will be prolonged accordingly.
Averaging can be performed in different ways, depending on whether the Mean or Sum method has
been activated.
If you are using the
Mean
method, the image information is generated by adding up all images and then
calculating the mean value.
In the
Sum method, the intensity values of all images are added up, without a mean value being
calculated.
Fig. 137 Acquisition Mode - Frame with
Camera tab selected in the Imaging
Setup tool