Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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5.3.8 Navigation – Focus
The function of the Focus tool allows you to
position the focus in Z direction.
•
Press the Focus tool tab to open the Focus tool
(Fig. 304).
Press anywhere outside the window to close the
tool box.
The following functions are available:
Zero Position display field (only available if Show
all
is checked): Displays the current z-position. L
indicates load, H home position. The arrows will
move the focus continuously u and down as long
as pressed.
Step Size [µm]
allows you to enter the steps the
focus will move.
Z-Position box displays the actual z-position. The
arrows will move the focus with a step size defined
in
Step Size [µm] .
Stop
button will interrupt an active z-movement.
Work button: Moves the stage / nosepiece back to
the Work position. This is the position last set
before the
Load button was pressed.
Load button: Lowers the stage / nosepiece to a default position make it easier for you to change the
sample carrier (or objective).
Zero Position Manually button will set the current z-position to zero.
If a z Piezo-stage insert is available the Piezo field will be displayed. The Piezo Position [
µ
m] box
displays the actual z-position of the Piezo-stage insert. The arrows will move the focus with a step size
defined in
Step Size [µm]
. Pressing the
Levelling
button will move the middle position of the z Piezo-
stage to the current z-position of the microscope stand's nosepiece.
Mark position button will store the current z-position.
Reuse #.# button will move to the z-position that was defined by Mark position . The z position number
#.#
will appear only, when
Mark position
has been pressed and indicates the z coordinate of the
present position.
The Z-position stored by activation of the Mark position button is a relative parameter and is only
valid during the current session. Accordingly, this position can be approached in a defined way only
during this session via
Reuse .
+200 µm
button: Moves the Z drive for +200 µm per mouse click upwards.
-200 µm button: Moves the Z drive for -200 µm per mouse click downwards.
If the sample is just a solution without structure, one cannot "focus" the instrument by looking at sample
features. Cover slip reflection has to be used instead to find the glass- / solution interface. The position of
the glass surfaces is detected most conveniently via line scanning.
Fig. 304 Focus tool