Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
94 000000-2071-464 10/2014 V_01
•
Use the slider to set the utilizable laser intensity
(recommendation: start at 1 %).
−
The transmittance of the Acousto-Optical
Tunable Filter (AOTF) changes accordingly.
This allows adapting the laser intensity very
sensitively to the job.
−
No more than 8 excitation laser lines
(wavelengths) can be active at the same
time. If more than eight laser lines are
connected to the system the Switch button is
active and the required laser lines (if not
displayed) must be allocated.
•
For this purpose, click on Switch and select the
required laser line that should be switched to
the one not yet displayed.
Multiple Tracks Configuration
The sequential imaging of tracks permits several tracks to be defined as one configuration for the scan
procedure, to be stored under any name, reloaded or deleted (see also section Acquisition Parameter –
Channels ).
The multiple tracks can either be configured manually one after the other (identical to a single track) and
then stored as acquisition parameters, or already existing acquisition parameters can be used and
changed as required.
It is also possible to load already stored track configurations (single tracks) in a recording configuration
(Available in the
Channels tool window).
The setup is done using both the
Channels
tool and
Imaging Setup
tool.
In the Imaging Setup tool chose Channel Mode as the mode for image acquisition and define the
hardware parameters like Laser line, laser attenuation, main and secondary dichroic beam splitters, and
emission filters for the first track as described above. Ideally the parameters should be stored as a
Track .
Add a second
Track
within the
Channels
tool. Make sure it’s selected (highlighted) before switching to
the Imaging Setup tool. Again set the hardware parameters in this case for the second track, and store
them under a different name as a Track in the
Channels
tool. If more than two tracks are requested,
repeat this procedure accordingly. Finally the total combination of tracks is stored as imaging
Configuration in the Channels tool.
When storing the acquisition parameters all the settings of the Imaging Setup plus Gain
(Master), Digital Offset, Digital Gain and Data Depth (see section
Acquisition Parameter –
Channels ), the scan parameters like Frame Size , Zoom, Rotation & Offset , and Scan
Direction
parameters (see section
Acquisition Parameter – Acquisition Mode
) are stored.
Fig. 113 Laser line selection and attenuation