Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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Activation/Deactivation of Channels
•
To activate a channel for imaging, check the check box
next to channel icon. To deactivate the channel uncheck
this box.
•
Use Channel T-PTM (transmission channel) to acquire an
image with transmission illumination using any laser for
excitation. This image will display the differential
interference contrast (DIC) of the specimen if the
according optical hardware for this contrast is put into
the beam path in front of the detector. Check the
manual of the microscope for setting DIC optics. Be
aware that the laser light is already polarized and
therefore a polarizer device is not necessary for
transmission imaging. This imaging mode allows
displaying a DIC image simultaneously to the
fluorescence signal of the specimen.
•
To assign a specific color to the image of a channel click
onto the
color triangle and select a color from the
drop down menu (Fig. 112).
•
To set the dye click onto the dye
triangle and select it from the drop down menu.
Reflector Turret
The filter wheel FW1 is the reflector turret of the microscope that holds push-and-click filter cubes and
fixed beam combiner cubes. When using the LSM for imaging the filter wheel is automatically set to an
empty position if not otherwise defined or set by loading an imaging configuration. Standard
fluorescence filter cubes are used for observation of the specimen with the eye or with the camera. A
click on the icon opens a drop down menu from which the desired filter can be chosen.
Stage / Focus
This icons provide access to the control for the focus (position of the specimen in Z) and the stage
(position of the specimen in X and Y). But detailed description is given in section
Focus Control and
section Stage Control .
Setting of Laser Line and Laser Attenuation
The Laser icon provides access to the control of the laser lines and their attenuation (Fig. 113). Activate a
laser line by checking the according box. This opens the attenuation slider and input box, which allows
setting the desired laser power. Make sure the laser line is indicated as active with a warning triangle. If
this is not the case, go back to the
Laser tool and switch on the laser.
Fig. 112 Drop down menu for
selection of the channel
color