Zeiss LSM 880, Operating Manual

Page: 531 / 690

CHAPTER 1 - SYSTEM OPERATION
LSM 880 Center Screen Area / Image Containers - Display and Image Analysis ZEISS
10/2014 V_01 000000-2071-464 525
Translation G
d
(
ξ
,
ψ
) describes the translational diffusion term of the model function. To open the
Translation
settings press the
Settings
button (Fig. 742).
Fig. 742 Settings for the Translation term
If the
Fractional intensities
box is checked, the
Molecular brightness
input boxes will become
available. Enter the absolute or relative molecular brightness of the molecules, which have to be
determined beforehand. If unchecked, molecular brightness will not be taken into account and the
Molecular brightness input box will not be displayed.
If the
Diffusion coefficient check box is ticked, the diffusion coefficient will be fitted, otherwise the
diffusion time. In case the box is checked, the
2 Photon
check box will be accessible. If ticked diffusion
time scales with 1/8
th
of the diffusion coefficient, if un-ticked with 1/4
th
. The 1/e
2
radius of the laser beam
must be entered within the
ω
r
in
µ
m
input box, which is also displayed only in case the Diffusion
coefficient box by typing or using the arrows.