Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
298 000000-2071-464 10/2014 V_01
Manual
•
For manual processing, deactivate the Auto
checkbox (Fig. 394).
•
In the preview window you can check again
whether the right image type is selected.
Note that this preview will not show a full
SR processing result.
•
Use the strength slider to get a stronger or weaker
result as with the auto processing. You might use
the same values as used in the 2D preview
processing in the
Airyscan tab.
•
Press the Apply button at the top of the dialog.
Processing will start and might take a few minutes.
A new image will open with the result of the manual
processing (Fig. 394). All images in a time series or
Z-Stack will be processed.
Note that with 3D data you have the choice
of a 2D or a 3D processing by selecting the
method from the dropdown list (Fig. 395). A
3D processing will take longer but will result
in a higher resolution increase since also the
axial Airy data will be used for the
processing.
VP data processing
In case a VP mode image was selected, the manual
processing slider will function for virtual pinhole
parameter adjustment.
This allows to adjust the confocal sectioning after
acquisition by binning the respective airy rings
together.
•
Press the Apply button at the top of the dialog.
Processing will start and might take a few seconds.
A new image will open with the result of the selected
sectioning. All images in a time series or Z-Stack will
be processed.
Fig. 394 Airyscan Processing tool:
Method Parameters, Manual
Fig. 395 Airyscan Processing tool:
Method Parameters, 2D, 3D