LSM 880
Left Tool Area and Hardware Control Tools
ZEISS
10/2014 V_01
000000-2071-464
111
Line Mode
In
Line
mode, fluorescent or reflected light along a
straight or freely definable line is displayed in the
form of an intensity profile. The line is scanned
pixel by pixel. The laser beam is moved over the
specimen along a line.
The following parameters for scanning a line can
be defined:
Objective, Frame Size, Speed
,
Bit Depth, Scan
Direction, Averaging and
Zoom including
rotation and offset
These functions operate as described in section
Frame size
in this context
sets the length of the line in x direction. The width
of the line is one pixel.
Note: A width larger than one pixel is defined as a
frame.
If
Line Mode
has been selected, the
Line Select
button appears on the bottom of the
Acquisition
mode
tool. It permits positioning of the line to be
scanned as required within the
Image
(Frame in
XY-plane).
A frame will be scanned and the currently selected
scan line and its intensity profile will be displayed.
In the
View Control
the
Line–select
tools are
available.
The line arrow
is active by default and allows using the mouse to draw a straight line in the image at
the position where the line scan should be performed.
Alternatively the scan line
can be defined as a free shape curve (spline).
The arrow selection
is used to move the drawn line within the image.
The standard controls allow defining the color and width of the appearance of the line overlay in the
image.
Once a straight line is drawn in the image, the relevant intensity profile along the drawn line will be
displayed. In the
Acquisition Mode
tool Zoom, Rotation and Offset values are updated.
•
When the
Line Select
button is hit again, a frame will be scanned in such a way that the selected line
lies exactly in the center of the Y-axis again and is parallel to the X-axis.
The position and size of the Line (rotation and offset) can also be changed directly in the
Scan
Area
panel of the
Acquisition Mode
tool.
In the
Line
mode, Line Stacks can also be recorded over a defined period of time.
•
When drawing a free shape curve (spline) in the
Image
using the mouse, the first click sets the
starting point; each further click adds a line segment. A click with the right mouse button ends the
line definition.
The scanner represented by a white line immediately begins with the on-line tracing of the defined free
shape curve. The laser excitation remains inactive in this process.
Fig. 138
Acquisition Mode – Line with LSM
tab selected in the Imaging Setup
tool
Summary of Contents for LSM 880
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