Zeiss LSM 880, Operating Manual

Page: 406 / 690

CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
400 000000-2071-464 10/2014 V_01
•
Click the LSM
Continuous
action button to
start a continuous scan. Optimize the pinhole
position in X, Y and Z (Z only for PH1) relative to
the PMT using the
Position X [µm]
,
Position Y
[µm] and Position Z [µm] sliders to maximum
image brightness (see Fig. 557).
•
The collimation for the visible laser lines (VIS
range = 458 to 633 nm) is preset and cannot be
changed.
•
You have access to the collimators for the inVIS
lasers = UV (355 nm), 405nm, 440nm and NLO
(multiphoton lasers). Each coupling port is
equipped with a motorized collimating optics.
Select the collimator for the port with the drop
down list next to
Name (Fig. 558). The
collimators name is then displayed next to the
drop down list and in the tool tip of the text.
•
Moving the Position [mm] slider in the
Collimators
panel allows the collimator to be
adjusted to maximum image brightness.
Optimum collimator adjustment obtained in this
way can be stored by clicking the
Current Pos
button. The Collimators are pre-set from the
factory and care should be exerted by changing
its position.
•
Collimator positions are stored for each combination of wavelength(s) and objective. This allows to
optimize settings for excitation wavelength combinations when working with lasers in the inVIS (UV or
NLO) and VIS range, or for combinations of IR lines (NLO). The settings are mainly influenced by the
chosen objective.
•
Collimator settings for NLO lasers are set only for one wavelength when the system is installed. Store
additional values if necessary for other objective / wavelength combinations by clicking
Store Current
Pos .
•
Stored collimator positions are automatically set when changing wavelengths and/or objectives.
Fig. 557 Manual pinhole adjustment
Fig. 558 Adjust Pinhole and Collimator tool
–
Collimators panel