Zeiss LSM 880, Operating Manual

Page: 336 / 690

CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
330 000000-2071-464 10/2014 V_01
5.4.14.4 PALM – PALM
The PALM function allows setting filters for peak
finding and choosing a fit model for the
localization calculations.
Select the
PALM tab to select the PALM
Processing tool (Fig. 431). Activate the image you
want to process in the image container and press
the
Select
button to load the image. Then expand
the
Method Parameters
tool window to have
access to the Peak Finder and Localizer
(Fig. 440).
You have to select the subdirectory
PALM
tab in order to have the PALM functionalities
available. The directory
PALM tab will not
give access to the PALM tools.
Pressing the Settings Default button will reset all
filter and parameter settings to the default values.
Pressing the
Settings Reuse
button will recall the
parameter setings of the actively selected image.
The
Channel
drop down menu allows you in a
multi-channel experiment to apply different
settings to different channels, which are all listed
(Fig. 441). If you select
All , all channels will be
fitted the same way. If you select a channel, and
change settings, these setting will apply only to
this channel. Note, even if only one channel is
selected, every channel will be fitted according to
the last settings.
The
Settings
drop down menu (Fig. 442) will
allow you to define how peak events will be fitted.
You have three options:
−
Discard overlapping molecules
(single
emitter): only single emitters will be taken
into account and fitted, whereas all multi-
emitter events will be discarded.
−
Ignore overlap
(ignore emitter): all single-
and multi-emitter events will be taken into
account and fitted as single emitter events.
Fig. 440 Processing – PALM – PALM
Fig. 441 Channel drop down menu of a dual
channel data set
Fig. 442 Settings drop down menu