Zeiss LSM 880, Operating Manual

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CHAPTER 5 - MULTIPHOTON LASER SCANNING …
LSM 880 Non Descanned Detection (NDD) ZEISS
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Fig. 11 Example for a filter equipment of a four channel NDD set up on Axio Observer starting with
an NDD module type C or D
For LSM NLO systems on Axio Imager (NDDs are only available for reflection) and Axio Examiner a
total of 5 detectors can be mounted onto the ports starting with a two channel module of type A or B.
The filter arrangement could be as described in Fig. 12. As the dichroic filters are also long pass filters
channel 1 always needs to see the red part of the spectrum followed by the blue part of the spectrum
within the required detection range. If more than 4 channels are required in reflection, a fiber coupled
illumination system (X-Cite 120 or HXP 120) instead of a standard HBO lamp is required.
Fig. 12 Potential filter equipment for a three channel NDD set up on Axio Examiner starting with an
NDD module type A or B
Adding NDD channel 3, 4 or 5 to an existing set up does require rearranging and swapping of individual
filters within and between single P&C cubes for the systems with Axio Examiner or Axio Imager.