Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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Using the channel alignment tool for drift corection
The following procedure can be used to remove lateral
drift in an inage:
•
Duplicate the time series. Select the time series in the
processing tool
Copy under the submenu
Duplication
(Fig. 507). Press
Apply
to obtain a copy
from your time series.
•
Generate reference image. Using the Processing tool
Copy select the duplicate time series in the submenu
Subset . Using Time select your reference image, for
example the first image or the center image, by
choosing for the
Start
and
End
value the same
number (Fig. 508). Press Apply to extract that image
from the time series.
•
Generate reference time series. In the Proceesing tool
Modify
choose under
Modify mode
the entry
Make
time series . Select the exctracted image. Enter under
Number of times
and
Time intervals
the numbers
from your original time series (Fig. 509) and press
Apply . The result will be a time series with the same
image at each time point.
Fig. 507 Methods Parameter of the
Copy Duplicat Processing tool
Fig. 508 Methods Parameter of the
Copy Subset Processing tool
Fig. 509 Methods Parameter with
Make time series selected in
Modify mode