Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Center Screen Area / Image Containers - Display and Image Analysis ZEISS
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Slightly reducing the SR filter strength will result in
an optimal result (Fig. 625).
Typically, this position is 0.3 to 0.5 units higher
than the Auto default value.
Processing Algorithm and Wiener filter (processing or filtering strength)
The image in a confocal system is the result of the convolution of the object by the effective PSF in the
spatial domain. Noise will add to the image. Because each detector element in Airyscan acts as an
independent pinhole, the image formed by each detector element will be a convolution of the object by
the effective PSF of each element plus added noise (equation 1). In the frequency domain, the Fourier
transform (FT) of the image of each detector element will be the product of the object spectrum with the
effective OTF of the respective detector element plus added noise spectrum (equation 2).
By inference, if the image is deconvolved by the effective PSF, one should be able to reconstitute the true
object structures. Likewise, if the image spectrum is divided by the effective OTF, one can obtain the
object spectrum that will be inversely Fourier transformed (FT-1) to obtain the true object. To reconstitute
the object spectrum one will have to weigh the contribution of each detector element to the image and
deconvolve the sum of each detector element (equation 3). This is handled conveniently by a linear
Wiener deconvolution, which takes noise into account (equation 4).
Fig. 625 Optimal result