Zeiss LSM 880, Operating Manual

Page: 283 / 690

CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
10/2014 V_01 000000-2071-464 277
(3) Pulse distance histogram
This diagram shows the frequency plotted against the times elapsed between two subsequent pulses (or:
photons recorded from the detector). To obtain this histogram, the time distance between two photons
is evaluated from the raw data.
You can zoom in the image by pressing the left mouse button and drawing a rectangle of the area you
want to zoom in. If the button is disengaged, the zoom image is displayed.
By clicking the right mouse button you can choose
between different options in the
Pulse Distance
Histogram context menu (Fig. 369).
•
Reset diagram zoom will reset any zoomed
image.
•
Copy text to clipboard will copy the diagram
coordinates into the clipboard, from which they
can be pasted into other programs like Excel.
Write text to file
will store the diagram
coordinates in a .txt file. You will be prompted
to choose a name and a folder before saving.
•
Choosing Abscissa logarithmic or Ordinate logarithmic will display the abscissa or ordinate in a
logarithmic scaling.
•
The
line width
allows you to select the thickness of the graph. 6 settings are possible.
(4) Photon counting histogram
This diagram (also called Photon Counting Histogram) shows the frequency plotted against the photon
number in a certain time bin. To obtain this histogram, the number of pulses (or: photons recorded from
the detector) in a moving time window are recorded and included in a histogram. You can determine the
binning time in the
Measure
tool in
Processing
under PCH or if you load a raw data file with the
Reload button.
You can zoom in the image by pressing the left mouse button and drawing a rectangle of the area you
want to zoom in. If the button is disengaged, the zoom image is displayed.
By clicking the right mouse button you can choose
between different options in the
Pulse density
Histogram context menu (Fig. 370).
•
If you have zoomed in, you can go to the
original image size by choosing the
Reset
diagram zoom
option.
•
Copy text to clipboard
will copy the diagram
coordinates into the clipboard, from which they
can be pasted into other programs like Excel.
•
Write text to file
will store the diagram
coordinates in a .txt file. You will be prompted
to choose a name and a folder before saving.
Fig. 369 Pulse Distance Histogram context
menu
Fig. 370 PCH context menu