Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Center Screen Area / Image Containers - Display and Image Analysis ZEISS
10/2014 V_01 000000-2071-464 507
Thresholds tab (Fig. 702):
The threshold for the image analysis can be set
manually using the slider or editing text box next
to
Donor
,
Acceptor
,
FRET
or
All
, where
All
moves all sliders to the same value. Alternatively,
the threshold can be set by defining and enabling
a background region in the FRET tab. The values
for the thresholds are either displayed as grey value
levels (
Raw data ) or Normalized to the value 1.
Settings tab (Fig. 698 and Fig. 703):
This tab provides access to the definition of the
parameters used for the image analysis.
The
General
(Fig. 698) tab applies to both
methods, Acceptor Photobleaching and Sensitized
Emission.
If checked the listed options will be taken into
account for the analysis of FRET.
The
Sensitized Emission
(Fig. 703) tab sets
additional Truncation parameters for each method
to analyze the Sensitized Emission experiment such
as the choice for
No truncation
,
Remove
maximum or the truncation of values above 1
(<=1), 2 (<=2) or 3 (<=3).
For the Fc analysis method, the option
Don’t
show negative values in the Fc image
can be
selected using the check box. With the FRET-N
normalization
slider and text box a multiplication
factor for the calculated FRET-N values can be set.
Fig. 702 FRET View Options Control Block,
Thresholds tab
Fig. 703 FRET View Options Control Block,
Settings tab, Sensitized Emission