Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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Delete Image
Single images or series of images can be deleted from
the selected image series (input image) acquired as
time series or Z-Stacks or combinations of those.
The
Select
button selects the active image in the
Image Display as the Input Image to be processed.
In the tool-specific settings panel, the parameters for
the interpolation procedure are set.
Opening for example the x-subset panel (Fig. 537)
brings up three sliders to set the
Start and End
coordinates of the new data set.
Pressing the
Mouse click button selects the
respective coordinate by clicking on the original image
in the
Preview View
in the image display area
(Center Screen Area). The Step coordinate determines
how many data points in the respective dimension are
dropped out. For example: setting
Step to 3 takes 1
and drops 2 pixels along the respective dimension,
reducing the data to 1/3. The scaling is calculated to
remain correct in the resulting image.
Subset
The
Subset
function is a set of tools to truncate a
multidimensional data set in all available coordinates
to the desired size (Fig. 538).
These subset panels are available for all coordinates of
the selected image (x, y, z, t, view, illumination). The
panel
Selection for all visible coordinates is shown
in Fig. 539. It always applies only to those coordinates
open within the subset-panel (above).
The Select by mouse click in image option allows one
to click in the Image Display and the respective
coordinates are set (if displayed in the image: x, y in
the 2D view, z, view and illumination in the gallery
view, etc.).
The
assign from viewer
selects the coordinate from
the slider in the Dimensions View Options control
block.
Fig. 537 Subset tool with loaded
Multiview (Lightsheet Z.1) image
Fig. 538 Subset panel for x-coordinate
Fig. 539 Subset panel for "visible"