Zeiss LSM 880, Operating Manual

Page: 382 / 690

CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
376 000000-2071-464 10/2014 V_01
To generate the maximum intensity projection image either the
Front View
or the
Side View
button
can be pressed. In the resulting maximum intensity projection image, each view is handeled as a channel
(e.g. Cam1-V1, Cam1-V2) of the image, a color can be assigned to each channel and it can be turned on
or off in the
image control area (Fig. 529).
Be aware, the calculation of the maximum intensity projection of the Front View or Side View
will take some processing time, depending on the size of the used dataset.
It is of no consequence with which of the maximum intensity projections the process is started. In case of
using the
Front View button, the maximum intensity projection is calculated along the z-axis and the x,y
axis are displayed in the resulting image (Fig. 530). The
Side View
button results in a maximum intensity
projection along the x-axis, displaying the z- (horizontal) and y- (vertikal) axis. The maximum intensity
projection image is opend in a new
image window , named Filename_MIP.czi. The following steps are
done on the basis of the maximum intensity projection, hence in this
image window
.
Fig. 529 Interactive Registration – Views into
Channel conversion of the maximum
intensity projection image
Fig. 530 Interactive Registration – Maximum
intensity projection (Front View) image
with red cross overlay for view rotation
(views Cam1-V1, Cam1-V3 and Cam1-V5
are visible in the image)