Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
324 000000-2071-464 10/2014 V_01
During data recording with the
Illumination
method, masks are applied to protect the sample
from laser irradiation. The respective ROIs are
displayed in an additional image (Fig. 428).
The calculation of the resulting image follows the
same procedures as for the sampling mode.
However, the regions, which are excluded from a
second and/or third illumination step are divided by
2 instead of 3.
After acquisition, the grey dynamics of the
resulting image are not automatically compressed
to match the range of the output display.
Use the
Gamma and Contrast sliders of the image
display (Fig. 429) to adjust the image.
For manual adjustment, a result can be achieved
which fits the individual requirements, without
affecting the actual raw data stored with the
image.
Fig. 428 HDR image display after using
Illumination mode
Fig. 429 Image adjustment of the HDR image (left side without, right with HDR)