Zeiss LSM 880, Operating Manual

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CHAPTER 2 - MACROS AND VISUAL BASIC
ZEISS Visual Macro Editor LSM 880
22 000000-2071-464 10/2014 V_01
This action block defines a mask (image region with defined properties) derived
from an image window. The image can be a newly acquired or imported image.
The properties of the mask based on the image data can be selected according
to the following properties:
−
Scan regions(s): The mask is based on the scan region(s) defined in the
ScanControl
.
−
Bleach region(s): The mask is based on bleached region(s) defined in the
BleachControl .
−
Image overlay: The mask is based on image overlay regions.
−
ROI list entry: The mask is based on ROIs listed in Edit ROI control window.
−
Interactive overlay: The mask is defined using overlay regions which are
defined while the macro is executed.
−
Interactive histogram: The mask is defined via histogram settings
(min/max) interactively defined while the macro is executed. This works in
conjunction with ROI selection (interactive or stored) as described above.
−
Region index: Index of ROI (i.e. ROI number) if for example multiple bleach
regions are active.
−
Channel: Image channel which is used to derive the mask parameters
from. This works in conjuction with interactive settings or loaded images.
−
Intensity range: Defines the intensity range which is applied for mask
definition.
−
Lower threshold: Defines lower intensity threshold which is applied for
mask definition. This threshold can be an absolute number or a floating
point.
−
Upper threshold: Defines upper intensity threshold which is applied for
mask definition. This threshold can be an absolute number or a floating
point.
Hardware Control Action Blocks
If a motorized stage is available the movement of the stage can be set up as
part of the program flow. The stage can be moved to an absolute position
which can also be taken from the actual stage position using the Read back
function or typed in individually. Using a relative position will use the current
stage position as relating position. The values have to be typed in.
It is also possible to choose the position from the
M
ark and Find
position list
which must have been set up in the stage control menu to be available.
The movement of the focus can be set up as part of the program flow. The
focus can be moved to an absolute position which can also be taken from the
actual focus position using the Read back function or typed in individually.
Using a relative position will use the current focus position as relating position.
The values have to be typed in. For large travel ranges which exceed half of the
objectives working distance a warning can be displayed.