Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Center Screen Area / Image Containers - Display and Image Analysis ZEISS
10/2014 V_01 000000-2071-464 455
By means of the
Depth Coding
function
(Fig. 641), the Z-information contained in a
sequence can be colored with the colors of the
rainbow, in which case "blue" stands for higher
values and "red" stands for lower values.
The
3D Textures option can be used for faster
and better rendering of transparency renderings.
Note that this function is only available in
Transparency and Maximum Intensity
Render Modes.
6.9.3 Maximum Mode
Clicking on the button switches to
Maximum projection
mode, which calculates a three-
dimensional view of the data by only showing pixels of the highest intensity along the projection axis.
This view is particularly useful for displaying three-dimensional images in two dimensions, e.g. in
publications, and guarantees a maximum possible degree of transparency. The display in this mode can
be calculated by the main processor (CPU) of the computer (Maximum basic) or the graphics card
(Maximum advanced) in OpenGL mode.
Note that this is a rendering. If desired 1:1 Maximum Intensity Projections can also be done in
the Processing
tab in Maximum Intensity Projections (see section Processing – Maximum
Intensity Projection
). With these Images exact Intensity Measurements can be performed.
Maximum Basic Maximum Intensity CPU based
Maximum Advanced Maximum Intensity GPU based
6.9.4 Surface Render Mode
In the Surface mode (Fig. 642) grey values in the
image are computed as solid surfaces (also known
as Iso surfaces) instead of soft transparent shades.
This display mode is useful to focus the attention
on certain structures (e.g. plant cell walls, nucleoli,
intracellular vesicles) while potentially hiding
additional structures deeper within.
In this mode you also can select between Voxel
(CPU) [basic] and OpenGL (graphics card)
[advanced] display.
The [full resolution] projection is based on a high
precision calculation method for 3D information on
the basis of triangles with maximum resolution.
Fig. 641 3D toolbar – Transparent – Depth
Coding
Fig. 642 3D toolbar – Surface