Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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5.2.3 Action Buttons
Action buttons provide control for how the image is acquired (Fig. 76).
Fig. 76 Action buttons
They have the following functions, regardless of the imaging mode:
AF Find Focus
triggers a frame z-Scan. The system selects the z-position associated with the frame of
highest intensity or contrast. AF Find Focus can be disrupted by pressing the Stop button, to which the
Snap
button changes upon activation (Fig. 77).
Fig. 77 Action buttons – AF Find Focus button active
Set Exposure
optimizes the gain and offset settings (PMT only, for cameras only the exposure time will
be adjusted) of all active channels and tracks dependent on the given laser power. Set Exposure can be
disrupted by pressing the Stop button, to which the Set Exposure button changes upon activation
(Fig. 78).
Fig. 78 Action buttons – Set exposure button active
Live uses the maximum speed for a given image frame (and zoom setting) for all active channels. It wil
set the resolution to 248 x 248 for the Andor SIM camera, 256 x 256 for the Andor PALM camera and
512 by 512 for single point detectors (PMT, GaAsP). To disrupt
Live acquisition, the Stop button has to
be pressed into which the
Live
button changes after its activation (Fig. 79).
Fig. 79 Action buttons – Live button active