Instruction LSM880 short 2017-12-26.docx 2017-12-26
LSM 880 quick operation manual
Switch on
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Make sure room temperature is 22-24°C.
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If necessary turn on air condition
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In the main switch box turn on the following switches 1
2
3 (do not touch the key)
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Turn on the computer and log in
User name: multilabs
Password: 123456
Position
Objective lens
Magni-
fication
NA
coverslip
Working
distance
Immersion
Parfocal length
2
Plan Apochromat
x10
0.45
0.17 mm
2 mm
Air
45 mm
3
Plan Apochromat
x20
0.8
0.17 mm
0.55 mm
Air
45 mm
4 (bombila)
W Plan Apochromat
x20
1.0
0
1.8 mm
Water dipping
75 mm
1+ adaptor
W Plan Apochromat
x40
1.0
0
2.5 mm
Water dipping
45 mm
1+ adaptor
Plan Apochromat
x63
1.4
0.17
0.19 mm
Oil
45 mm
Software and acquisition
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Log into BookitLab and activate your reservation to start Black Zen 2.3
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To acquire images click Start System
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In the acquisition tab
Laser tab
Switch on the lasers, (The Argon laser should be turned on by Standby
ON. Please wait 5' minutes until
the laser stabilizes, its status should be optimal in the properties tab).
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Sample mounting and viewing
Insert the slide or dish in the holder.
To change objective turn the black wheel to point up to release the objective turret. Turn manually to the
required objective, lower the objective
by pointing the black wheel in the objective turret down, (if an
objective needs to be replaced please ask the microscopy staff).
In the software go to Locate tab.
On the microscope’s right side choose VIS
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Transmitted light
Click the transmitted light lamp icon, click ON and set desired brightness (or via the microscope).
Set the filter turret position to DIC and the condenser below the stage to II or III according to the objective
lens (see objective lens info on touchpad).
Set up Kohler illumination.
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Reflected light (fluorescence)
Open fluorescence shutter
Set the filter turret to the desired position
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Image Acquisition
On the microscope’s right side choose LSM
In Zen, Acquisition tab:
Load a saved configuration from the Experiment Manager or open an image and below the image click
Reuse
Select Show all tools and open the following tabs:
